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hClaudin-4 MAb (Cl 3 (25 UG)

hClaudin-4 MAb (Cl 3 (25 UG)

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品牌: R&D Systems
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来源宿主:
Mouse IgG2a
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
claudin 4, Claudin4, Claudin-4, CLDN4, Clostridium perfringens enterotoxin receptor 1, Clostridium p
宿主
Mouse IgG2a
克隆号
382316

Scientific Data

Immunocytochemistry Claudin-4 antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC).View Larger

Claudin‑4 in PC-3 Human Cell Line. Claudin-4 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and HLDM-2 human Hodgkin's lymphoma cell line (negative staining) using Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB42191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Human Claudin-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin-4 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 382316
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-4
Met1-Val209
Accession # NP_001296
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

背景
别名
claudin 4,Claudin4,Claudin-4,CLDN4,Clostridium perfringens enterotoxin receptor 1,Clostridium perfringens enterotoxin receptor,CPE-R,CPE-RCPETR,CPE-receptor,CPETR1,hCPE-R,WBSCR8,WBSCR8CPERCPETR1claudin-4,Williams-Beuren syndrome chromosomal region 8 protein
背景

Background: Claudin-4

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up‑regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Entrez Gene IDs
1364 (Human); 12740 (Mouse); 304407 (Rat)
Alternate Names
claudin 4; Claudin4; Claudin-4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-RCPETR; CPE-receptor; CPETR1; hCPE-R; WBSCR8; WBSCR8CPERCPETR1claudin-4; Williams-Beuren syndrome chromosomal region 8 protein
制备和贮存
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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货号:
MAB42191-SP
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询价
25UG
100UG
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