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Pro23-Ser439
Accession # Q6UWV6
Scientific Data
View LargerDetection of Human ENPP‑7/Alk‑SMase by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ENPP-7/Alk-SMase Monoclonal Antibody (Catalog # MAB4924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ENPP-7/Alk-SMase at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human ENPP-7/Alk-SMase Antibody Summary
Pro23-Ser439
Accession # Q6UWV6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Background: ENPP-7/Alk-SMase
ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is a 60 kDa GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%‑36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is down‑regulated in some human colorectal carcinomas. The 416 amino acid (aa) mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
参考图片
Detection of Human ENPP‑7/Alk‑SMase by Western Blot.
Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human ENPP‑7/Alk‑SMase Monoclonal Antibody (Catalog # MAB4924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ENPP‑7/Alk‑SMase at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.