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PBS pH7.4, 0.03% Proclin 300
ELISA
Sandwich ELISA
CLIA
Lateral Flow
Dot Blot
WB
IP
IHC-P
ICC
IF
ICFCM
FCM
20μl per 10^7 cells in 1ml volumemIHC
FcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
参考图片
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Goat Anti - Mouse IgG Alexa Fluor® 488 after incubated for 30 min on ice in Human Fc Receptor Blocker antibody at 1/50 (100 µg, 1x107 in 1 mL) dilution (Left) to block Fc receptors and non-specific protein compared with PBS (Right). Gated on total viable cells.
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Goat Anti - Rabbit IgG Alexa Fluor® 488 after incubated for 30 min on ice in Human Fc Receptor Blocker antibody at 1/50 (100 µg, 1x107 in 1 mL) dilution (Red) to block Fc receptors and non-specific protein. Compared with PBS (Black) or unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on total viable cells.