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Human GBA3 / CBGL1 Protein (His Tag)
Human GBA3 / CBGL1 Protein (His Tag)
品牌： Sino Biological
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对比
咨询分子量：
The recombinant human GBA3 consists of 480 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
纯度：
> 90 % as determined by SDS-PAGE
产品介绍
产品介绍
产品信息
抗原名称
glucosidase, beta, acid 3 (gene/pseudogene)
N端序列分析
Met 1
分子量
The recombinant human GBA3 consists of 480 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
生物活性
Measured by its ability to hydrolyze 4-methylumbelliferyl-β-D glucopyranoside.
The specific activity is >1,500 pmoles/min/μg
.
纯度
> 90 % as determined by SDS-PAGE
内毒素水平
< 1.0 EU per μg of the protein as determined by the LAL method
形式
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
表达系统
Baculovirus
应用
反应种属
Human
背景
别名
CBG Protein, Human; CBGL1 Protein, Human; GLUC Protein, Human; KLRP Protein, Human
背景
Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.
Full Name
glucosidase, beta, acid 3 (gene/pseudogene)
References
Yahata K., et al.,(2000), Molecular cloning and expression of a novel klotho-related protein. J. Mol. Med. 78:389-394.de Graaf M., et al., (2001), Cloning and characterization of human liver cytosolic beta-glycosidase.Biochem. J. 356:907-910.Berrin J.-G., et al.,(2002), Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides.Eur. J. Biochem. 269:249-258.
制备和贮存
保存方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
数据库链接
Accession
NP_066024.1
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