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Human LAP (TGF-beta 1) MAb (Clone 9005) (25 ug)

品牌： R&D Systems

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产品介绍

产品信息

荧光素标记

Unconjugated

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

CED， DPD1， LAP (TGFbeta 1)， LAP (TGF-beta 1)， LAP， TGFB， TGFB1， TGFbeta， transforming growth factor

宿主

Mouse IgG1

免疫原

Chinese hamster ovary cell line CHO-derived recombinant human Latent TGF‑ beta 1
Leu30-Ser390
Accession # P01137

克隆号

9005

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LAP (TGF-beta 1) Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAP (TGF-beta 1) and Latent TGF-beta 1 in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 9005

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Latent TGF‑ beta 1
Leu30-Ser390
Accession # P01137

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human LAP TGF-beta 1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human LAP TGF-beta 1 Biotinylated Antibody (Catalog # BAM2462)

Standard: Recombinant Human LAP (TGF-beta 1) Protein (Catalog # 246-LP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avian or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as Latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Entrez Gene IDs

7040 (Human)

Alternate Names

CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Airway Inflammation and Remodeling
Cytokines and Receptors in Angiogenesis
gamma delta T Cells
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
Platelet Cytokine and Growth Factor Receptors
T Cell Cytokine Signaling
TGF-beta Family
TGF-beta Family Ligands
Th17 Cells
Th22 Cells
Th9 Cells

应用

实验应用

ELISA Capture (Matched Antibody Pair)(2-8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.5-2.0 µg/mL )
ELISA Standard ( )

背景

别名

CED,DPD1,LAP (TGFbeta 1),LAP (TGF-beta 1),LAP,TGFB,TGFB1,TGFbeta,transforming growth factor beta 1,Latency-associated Peptide

背景

Background: LAP (TGF-beta 1)

Long Name

Latency-associated Peptide

Entrez Gene IDs

7040 (Human)

Alternate Names

CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

研究领域

Related Research Areas

Airway Inflammation and Remodeling
Cytokines and Receptors in Angiogenesis
gamma delta T Cells
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
Platelet Cytokine and Growth Factor Receptors
T Cell Cytokine Signaling
TGF-beta Family
TGF-beta Family Ligands
Th17 Cells
Th22 Cells
Th9 Cells

制备和贮存

溶解方法

Reconstitute at 0.5 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

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货号：

MAB2461-SP

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