Human Latent transforming growth factor beta binding protein 1 ELISA Kit

latent-transforming growth factor beta-binding protein 1 isoform 5

Intended Uses: This LTBP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LTBP-1. This ELISA kit for research use only, not for therapeutic or test applications! Principle of the Assay: LTBP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LTBP-1 antibody and an LTBP-1-HRP conjugate. The assay sample and buffer are incubated together with LTBP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTBP-1 concentration since LTBP-1 from samples and LTBP-1-HRP conjugate compete for the anti-LTBP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LTBP-1 from the sample, fewer sites are left to bind LTBP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTBP-1 concentration in each sample is interpolated from this standard curve.

Human

Quantitative Competitive

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

1.0 ng/mL

Human Latent transforming growth factor b binding protein 1 ELISA Kit

4052

Q14766 [Other Products]