Human Natural Killer Cell Markers Flow Cytometry Panel

Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.

The Human Natural Killer Cell Markers Flow Cytometry Panel can be used to detect human natural killer (NK) cells.

Human natural killer cells are CD45+CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic natural killer cells, while those with low CD16 expression are immature precursors and cytokine producers.

Product Usage Information

All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis.

Gating strategy for identifying human natural killer cells:

If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate based on CD45 expression, then gate on lymphocytes based on forward scatter and side scatter on CD45+ fraction. NK cells are CD3-CD56+, and these cells can further be analyzed for CD16 expression.

Specificity/Sensitivity

Species Reactivity:

Human