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Human Pluripotent Stem Cell 3-Color Immunocytochemistry Kit (1 Kit)
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咨询Human Pluripotent Stem Cell 3-Color Immunocytochemistry Kit Summary
To verify pluripotency in human stem cells using single-step ICC.
Key Benefits
- Defines pluripotency in the starting stem cell population
- Reduces experimental variation
- Utilizes single-step, fluorescent ICC staining of pluripotency markers
Why Verify the Pluripotency of a Human Stem Cell Population?
Maintaining and expanding healthy, undifferentiated stem cells in culture can be challenging if spontaneous differentiation occurs.
In addition, research studies using embryonic or induced pluripotent stem cells most often require weeks of cell culture before an experimental hypothesis can be tested.
Verifying stem cell pluripotency using 3-color ICC:
- Defines the starting stem cell population.
- Increases confidence in pluripotent status by combining 3 different fluorochrome-conjugated antibodies for simultaneous staining.
- Reduces variability within an experiment and between studies.
- Allows researchers to expand and study high quality, undifferentiated stem cell starting populations.
- Single-step staining ensures efficient use of time and reagents.
This kit contains the following fluorochrome-conjugated antibodies to verify human stem cell pluripotency:
- NL557-conjugated Goat Anti-Human SOX2
- NL637-conjugated Goat Anti-Human Oct-3/4
- NL493-conjugated Goat Anti-Human Nanog
Each antibody is supplied as a 10X stock; enough for 25 assays when used in a 50 µL staining volume per assay.
Specifications
Product Datasheets
Scientific Data
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Verification of Human Embryonic Stem Cell Pluripotency. BG01V human embryonic stem cells grown on Irradiated Mouse Embryonic Fibroblasts (Catalog # PSC001) were simultaneously stained with the following antibodies supplied in the Human Pluripotent Stem Cell 3-Color Immunocytochemistry Kit (Catalog # SC021): NL637-conjugated Oct-3/4, NL493-conjugated Nanog, and NL557-conjugated SOX2. The nuclei were counterstained with DAPI. The uniform expression of three stem cell markers increases confidence in the pluripotent status of this stem cell population. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Analysis of Human Stem Cell Pluripotency. iPS2 human induced pluripotent stem cells were grown on StemXVivo®Culture Matrix (Catalog # CCM013) in Mouse Embryonic Fibroblast (MEF) Conditioned Media (Catalog # AR005). The cells were simultaneously stained with NL637-conjugated Oct-3/4 (pseudocolored green) and NL557-conjugated SOX2 (red) antibodies, supplied in the Human Pluripotent Stem Cell 3-Color Immunocytochemistry Kit (Catalog # SC021). Co-staining reveals that some cells are negative for SOX2, suggesting that the population of Oct-3/4+cells is not uniform and some of the cells may be initiating differentiation. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.