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咨询Immunoprecipitation(25 µg/mL)
Ala20-Asp707 (Gln279Arg)
Accession # P14780
Scientific Data
View LargerDetection of Human Pro MMP‑9 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line stimulated with 100 ng/ml PMA for 24 hours under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Pro MMP-9 Monoclonal Antibody (Catalog # MAB9111) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Pro MMP-9 was detected at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human Pro MMP-9 Antibody Summary
Ala20-Asp707 (Gln279Arg)
Accession # P14780
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Immunoprecipitation(25 µg/mL)
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
参考图片
Detection of Human Pro MMP‑9 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line stimulated with 100 ng/ml PMA for 24 hours under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9111) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Pro MMP‑9 was detected at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.