Human Ribosomal Protein L6 (RPL6) ELISA Kit

60S ribosomal protein L6 isoform 1

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RPL6 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPL6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RPL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RPL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RPL6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human

0.156-10ng/mL

Sandwich

Tissue homogenates, cell lysates and other biological fluids.

0.059ng/mL

SHUJUN-2; TAXREB107; TXREB1; Neoplasm-related protein C140; Tax-responsive enhancer element-binding protein 107

6128

Q02878 [Other Products]