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hRUNX2 MAb (Cl 23290 (100 ug)
hRUNX2 MAb (Cl 23290 (100 ug)

hRUNX2 MAb (Cl 23290 (100 ug)

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品牌： R&D Systems
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来源宿主：
Rat IgG2b
实验应用：
Immunocytochemistry(8-25 µg/mL)
产品介绍
产品信息
荧光素标记
Unconjugated
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
Acute myeloid leukemia 3 protein， CBFA1， CBF-alpha-1， CCD1， CCDAML3， CLCD， Core-binding factor subun
宿主
Rat IgG2b
免疫原
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950
克隆号
232902

Scientific Data

Immunocytochemistry RUNX2/CBFA1 antibody in U2OS Human Cell Line by Immunocytochemistry (ICC).View Larger

RUNX2/CBFA1 in U2OS Human Cell Line. RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Human RUNX2/CBFA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RUNX2/CBFA1.
Source
Monoclonal Rat IgG2B Clone # 232902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RUNX2/CBFA1 isoform 2
Lys233-Tyr418
Accession # Q13950
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

组成成分
IGG-免疫球蛋白G型抗体
应用
实验应用
Immunocytochemistry(8-25 µg/mL)
背景
别名
Acute myeloid leukemia 3 protein,CBFA1,CBF-alpha-1,CCD1,CCDAML3,CLCD,Core-binding factor subunit alpha-1,core-binding factor, runt domain, alpha subunit 1,MGC120023,ML3,oncogene AML-3,OSF2,OSF-2,osteoblast-specific transcription factor 2,PEA2aA,PEA2-alpha A,PEBP2A,PEBP2aA,PEBP2-alpha A,polyomavirus enhancer-binding protein 2 alpha A subunit,runt domain, alpha subunit 1,runt related transcription factor 2,runt-related transcription factor 2,RUNX2,SL3/AKV core-binding factor alpha A subunit,SL3-3 enhancer factor 1 alpha A subunit,Runt-related Transcription Factor 2
背景

Background: RUNX2/CBFA1

CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation (1). The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities (2).

References
  1. Ducy, P. et al. (1997) Cell 89:747.
  2. Xiao, Z.S. et al. (1998) Gene 214:187.
  3. Sato, M. et al. (1998) Oncogene 17:1517.
Long Name
Runt-related Transcription Factor 2
Entrez Gene IDs
860 (Human)
Alternate Names
Acute myeloid leukemia 3 protein; CBFA1; CBF-alpha-1; CCD1; CCDAML3; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120023; ML3; oncogene AML-3; OSF2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2A; PEBP2aA; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit
研究领域
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

RUNX2/CBFA1 in U2OS Human Cell Line. RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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货号：
MAB2006
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