h/mNurr1 Aff Pur PAb (100 ug)

1/2

h/mNurr1 Aff Pur PAb (100 ug)

品牌： R&D Systems

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

来源宿主：

实验应用：

产品介绍

产品信息

荧光素标记

Unconjugated

纯化方式

Antigen Affinity-purified

抗原名称

human homolog of， NGFI-B beta， NOT， NR4A2， nuclear receptor subfamily 4, group A, member 2， Nurr1， o

宿主

Goat IgG

免疫原

E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
Val332-Lys558
Accession # Q06219

Scientific Data

Immunocytochemistry

View Larger

Nurr1/NGFI‑B beta /NR4A2 in HeLa Human Cell Line. Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

View Larger

Nurr1/NGFI‑B beta /NR4A2 in Mouse Brain (Hippocampus). Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Nurr1/NGFI‑B beta /NR4A2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
Val332-Lys558
Accession # Q06219

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Nurr1/NGFI‑B beta /NR4A2

Immunohistochemistry

5-15 µg/mL

Immersion fixed differentiated dopamenergic nuerons from D3 mouse embryonic stem cells

Immunocytochemistry

5-15 µg/mL

Immersion fixed HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Western Blot(0.1 µg/mL)
Immunohistochemistry(5-15 µg/mL)

背景

别名

human homolog of,NGFI-B beta,NOT,NR4A2,nuclear receptor subfamily 4, group A, member 2,Nurr1,orphan nuclear receptor NR4A2,Orphan nuclear receptor NURR1,T-cell nuclear receptor NOT,TINUR,transcriptionally inducible nuclear receptor related 1,Transcriptionally-inducible nuclear receptor,Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2

背景

Background: Nurr1/NGFI-B beta/NR4A2

Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.

Long Name

Nur-related Factor 1/Nerve Growth Factor Induced B beta/Nuclear Receptor Subfamily 4, Group A, 2

Entrez Gene IDs

4929 (Human); 18227 (Mouse)

Alternate Names

human homolog of; NGFI-B beta; NOT; NR4A2; nuclear receptor subfamily 4, group A, member 2; Nurr1; orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; T-cell nuclear receptor NOT; TINUR; transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor

研究领域

Related Research Areas

制备和贮存

溶解方法

Reconstitute at 0.2 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

AF2156

一键复制

询价

25ug

100ug

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价