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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the 6xHis epitope tag.
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 |
| Chromatin IP | 1:100 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
293T cells were either untransfected (left panel) or transfected with an His-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from cells and His-Tag (D3I1O) XP® Rabbit mAb, Stat3α (D1A5) XP® Rabbit mAb #8768, or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Western blot analysis of extracts from 293T cells, untransfected or transfected with 6xHis-Tag fusion protein 1 or 6xHis-Tag fusion protein 2, using His-Tag (D3I1O) XP® Rabbit mAb.
Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 mAkt1-Myc/His Transfection. cells using His-Tag (D3I1O) XP® Rabbit mAb #12698. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of His-Tag protein from transfected 293T cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or His-Tag (D3I1O) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using His-Tag (D3I1O) XP® Rabbit mAb.
Confocal immunofluorescent analysis of 293T cells transfected with a His-Tagged protein using His-Tag (D3I1O) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with a His-myc-Akt plasmid (green), using His-Tag (D3I1O) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.