His-Tag (D3I1O) XP ® Rabbit mAb

Confocal immunofluorescent analysis of 293T cells transfected with a His-Tagged protein using His-Tag (D3I1O) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析293T细胞，转染了His标签蛋白，使用的抗体是：His-Tag (D3I1O) XP® Rabbit mAb（绿色）。肌动蛋白纤维用DY-554 phalloidin标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Western blot analysis of extracts from 293T cells, untransfected or transfected with 6xHis-Tag fusion protein 1 or 6xHis-Tag fusion protein 2, using His-Tag (D3I1O) XP^® Rabbit mAb.Weatern blot分析293T细胞提取物，未转染（-）或转染了6xHis标签融合蛋白1或2，所用抗体为His-Tag (D3I1O) XP® Rabbit mAb。

Immunoprecipitation of His-Tag protein from transfected 293T cells using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or His-Tag (D3I1O) XP^® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using His-Tag (D3I1O) XP^® Rabbit mAb.免疫沉淀分析293T细胞中His标签蛋白，所用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 His-Tag (D3I1O) XP® Rabbit mAb (泳道3)。泳道1为10%的细胞提取物。采用His-Tag (D3I1O) XP® Rabbit mAb进行western分析。

Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with a His-myc-Akt plasmid (green), using His-Tag (D3I1O) XP^® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术分析293T细胞，未转染（蓝色）或转染了His-myc-Akt质粒（绿色）。使用的抗体是His-Tag (D3I1O) XP® Rabbit mAb。二抗是 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414。

293T cells were either untransfected (left panel) or transfected with an His-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ cells and 5 µl of His-Tag (D3I1O) XP^® Rabbit mAb, 5 µl of Stat3α (D1A5) XP^® Rabbit mAb #8768, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.293T细胞未转染（左）或转染了His标签的人Stat3载体（右），再由人Interleukin-6 (hIL-6) #8904处理（100ng/ml,30分钟）。染色质免疫共沉淀使用4 x 106的细胞中的染色质与5 µl的His-Tag (D3I1O) XP® Rabbit mAb，5 µl of Stat3α (D1A5) XP® Rabbit mAb #8768, 或 2 µl of Normal Rabbit IgG #2729，使用的是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集的DNA用实时定量PCR检测，使用SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, 和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样本中免疫沉淀下来的DNA与总染色质的量做对比。