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His-Tag (27E8) Mouse mAb

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His-Tag (27E8) Mouse mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

His-Tag

来源纯化

Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide.

宿主

Mouse

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:200

同种型

Mouse IgG1

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. The antibody may cross-react with a protein of unknown origin ~60-70kDa.

Species Reactivity:

All Species Expected

敏感性

Transfected Only

背景

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.A variety of plasmids contain DNA that encodes an amino-terminal tag cosisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1).As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1). 1.Kroll, D.J. et al. (1993) DNA Cell Biol 12, 441-53. 2.di Guan, C. et al. (1988) Gene 67, 21-30.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Immunoprecipitation of control lysate (lane 1) or C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb, followed by Western analysis with His-Tag Polyclonal Antibody #2365.

Western blot analysis of extracts from control (lane 1) or cells expressing C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb.

Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells, transfected with mAkt1-Myc/His (C-terminal Myc/His tag), using His-Tag (27E8) Mouse mAb #2366. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

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货号：

2366T

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20ul

100ul

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