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BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E

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BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E

品牌： BD Pharmingen

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产品介绍

产品信息

抗原名称

I-A/I-E

宿主

Rat BN x LEW IgG2b, κ

免疫原

Activated C57BL/6 Mouse Spleen Cells

简单描述

The M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens.

商品描述

M5/114.15.2 The M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens.

同种型

Rat BN x LEW IgG2b, κ

克隆号

克隆 M5/114.15.2 (also known as M5/114) (RUO)

浓度

0.2 mg/ml

产品详情

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Mouse (QC Testing)

目标/特异性

I-A/I-E

背景

别名

H-2I; I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloAgs; Ia Ag; M5/114; MHC II

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(7) 1. Bhattacharya A, Dorf ME, Springer TA. A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981; 127(6):2488-2495. (Immunogen: Immunoprecipitation). 2. Guo MW, Watanabe T, Mori E, Mori T. Molecular structure and function of CD4 on murine egg plasma membrane. Zygote. 1995; 3(1):65-73. (Clone-specific: Blocking). 3. Hattori M, Buse JB, Jackson RA, et al. The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex. Science. 1986; 231(4739):733-735. (Clone-specific: Flow cytometry). 4. Nelson AJ, Hosier S, Brady W, Linsley PS, Farr AG. Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro. J Immunol. 1998; 151(5):2453-2461. (Clone-specific: Immunofluorescence, Immunohistochemistry). 5. Shi Y, Kaliyaperumal A, Lu L, et al. Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain. J Exp Med. 1998; 187(3):367-378. (Clone-specific: Blocking). 6. Viville S, Neefjes J, Lotteau V, et al. Mice lacking the MHC class II-associated invariant chain. Cell. 1993; 72(4):635-648. (Clone-specific: Flow cytometry, Immunofluorescence). 7. Yamashita I, Nagata T, Tada T, Nakayama T. CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection. Int Immunol. 1993; 5(9):1139-1150. (Clone-specific: Blocking).

数据库链接

Entrez-Gene ID

111861

参考图片

Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains. Mouse spleen cells from either M5/114-negative SJL (Left Panel) or M5/114-positive BALB/c (Right Panel) mice were stained with Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E (Cat. No. 562367), PE Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878) and PE Anti-Mouse CD11b (Cat. No. 553311/557397/561689) antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-Ad and I-Ed MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on splenocytes from positive and negative mouse strains. Mouse spleen cells from either M5/114-negative SJL (Left Panel) or M5/114-positive BALB/c (Right Panel) mice were stained with Alexa Fluor® 647 Rat Anti-Mouse I-A/I-E (Cat. No. 562367), PE Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878) and PE Anti-Mouse CD11b (Cat. No. 553311/557397/561689) antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. The M5/114 monoclonal antibody detects I-Ad and I-Ed MHC class II alloantigens that are expressed on both B cells and macrophages. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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货号：

562367

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