The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.
The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filter. This dye has been exclusively developed by BD Biosciences and is up to seven times brighter than FITC with less spillover into the PE channel. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. It is not recommended to use BB515 in cocktails that include Streptavidin conjugates as it may cause high background.
商品描述
2G9
The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.
The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filter. This dye has been exclusively developed by BD Biosciences and is up to seven times brighter than FITC with less spillover into the PE channel. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. It is not recommended to use BB515 in cocktails that include Streptavidin conjugates as it may cause high background.
同种型
Rat DA, also known as DA/HA IgG2a, κ
克隆号
克隆 2G9 (RUO)
浓度
0.2 mg/ml
产品详情
BB515
The BD Horizon Brilliant™ Blue 515 (BB515) dye is part of the BD Horizon Brilliant™ Blue family of dyes. This dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 490-nm and an emission maximum (Em Max) of 515-nm. Driven by BD innovation, BB515 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 520-nm (e.g., 530/30-nm). BB515 reagents are significantly brighter than equivalent FITC or Alexa Fluor™ 488 reagents with less spillover into the PE detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BB515
Blue 488 nm
490 nm
515 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
I-A/I-E
背景
别名
H-2I; I-Ad, -Ed; I-Eb and I-Ek MHC class II alloantigens; Ia Ag; MHC II
研发参考(2)
1. Becker D, Mohamadzadeh M, Reske K, Knop J. Increased level of intracellular MHC class II molecules in murine Langerhans cells following in vivo and in vitro administration of contact allergens. J Invest Dermatol. 1992; 99(5):545-549. (Immunogen: Immunofluorescence, Immunoprecipitation).
2. Farquhar CA, Paterson AM, Cobbold SP, et al. Tolerogenicity is not an absolute property of a dendritic cell. Eur J Immunol. 2010; 40(6):1728-1737. (Clone-specific: Flow cytometry).
数据库链接
Entrez-Gene ID
14961, 14969
参考图片
Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on mouse splenocytes - Staining comparisons between BD Horizon™ BB515- and FITC-conjugated antibodies. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) antibodies, and either BB515 Rat IgG2a, κ Isotype Control (Cat. No. 564418; Left Panel), BD Horizon BB515 Rat Anti-Mouse I-A/I-E (Cat. No. 565254; Middle Panel), or FITC Rat Anti-Mouse I-A/I-E (Cat. No. 553623/562009; Right Panel). Two-color flow cytometric contour plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Mouse splenic leucocytes from SJL mice (H-2s haplotype) did not stain positively with the 2G9 antibody (data not shown). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on mouse splenocytes - Staining comparisons between BD Horizon™ BB515- and FITC-conjugated antibodies. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) antibodies, and either BB515 Rat IgG2a, κ Isotype Control (Cat. No. 564418; Left Panel), BD Horizon BB515 Rat Anti-Mouse I-A/I-E (Cat. No. 565254; Middle Panel), or FITC Rat Anti-Mouse I-A/I-E (Cat. No. 553623/562009; Right Panel). Two-color flow cytometric contour plots showing the expression of I-A/I-E MHC class II alloantigens versus CD45R/B220 and CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Mouse splenic leucocytes from SJL mice (H-2s haplotype) did not stain positively with the 2G9 antibody (data not shown). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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