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产品介绍
产品介绍
产品信息
抗原来源
Human

宿主
Rabbit

免疫原
Synthetic peptide corresponding to human KIT.

产品用途
Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.

简单描述
KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.

同种型
IgG

克隆类别
Polyclonal

纯度
Purified by immunoaffinity chromatography.

形式
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

应用
实验应用
E IC IF WB

推荐用量
Western Blot: 1:500-1:2000, For Western Blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.

反应种属
Rabbit

交叉反应性
Hu Mo

目标/特异性
Recognizes endogenous levels of total human KIT. Species Crossreactivity: Mouse

稀释度
Western Blot: 1:500-1:2000, For Western Blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100-1:500 ELISA (peptide): 1:20,000-1:40,000 Optimal dilutions to be determined by the researcher.
背景
别名
C Kit, c-Kit, c-Kit Ligand, CD117, Kit, Kit Ligand, KIT oncogene, KIT proto oncogene receptor tyrosine kinase, KIT_HUMAN, Mast cell growth factor receptor, Mast/stem cell growth factor receptor Kit, MGF, p145 c-kit, PBT, Piebald trait protein, Proto oncogene c Kit, Proto oncogene tyrosine protein kinase Kit, Proto-oncogene c-Kit, SCF Receptor, SCFR, soluble KIT variant 1, Steel Factor Receptor, Stem cell factor receptor, tyrosine protein kinase Kit, Tyrosine-protein kinase Kit, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

制备和贮存
保存方式
Storage_Temp:-20°C
Storage_and_Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
数据库链接
UniProt ID
P10721

SwissProt
P10721

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