IdeS Protease (Tag Free)

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IdeS Protease (Tag Free)

品牌： UA BIOSCIENCE

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参数

分子量：

38 kDa (Reducing)

纯度：

＞95% by SDS-PAGE

产品介绍

产品信息

耦联标记

Unconjugated

分子量

38 kDa (Reducing)

酶活定义

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.

纯度

＞95% by SDS-PAGE

性状

Lyophilized Powder

表达系统

E.coli

缓冲体系

PBS pH 6.6

组合货号

PBS pH 6.6

标签

No Tag

种属

Streptococcus pyogenes

电泳JSON

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\n2μg (R: reducing condition, N: non-reducing\ncondition).\n\n\n\n

"}]

背景

别名

IdeS、IdeS Protease、Immunoglubulin-degrading enzyme

背景

IdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcus pyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs

制备和贮存

保存方式

·12 months from date of receipt, -20 to -70 °C as supplied.

· It can be stored at 4-8℃ for one week after dissolution, under sterile conditions

· Please avoid repeated freeze-thaw cycles.

文献

[1] Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterization of the streptococcal endopeptidase, IdeS, reveals that it is a cysteine protease with strict specificity for IgG cleavage due to exosite binding.[J].Biochemistry, 2004, 43(49):15540-15549.

[2] Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615.

参考图片

2μg (R: reducing condition, N: non-reducing condition).

The results of 5μg rabbit IgG digestion separated under different quantity of IdeS Protease(Tag Free), The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).

M, marker;

lane 1 rabbit IgG;

lane 2 rabbit IgG add 5 units IdeS Protease (Tag Free);

lane 3 rabbit IgG add 10units IdeS Protease (Tag Free);

lane 4 rabbit IgG add 16.7 units IdeS Protease (Tag Free);

lane 5 rabbit IgG add 50 units IdeS Protease (Tag Free);

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货号：

UA070032-2000U

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2000U

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2mg

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当前规格1件起购

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