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N.A
37.7kDa
M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing\ncondition . The purity is greater than 95%.
"}]IdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcuspyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.
N.A
12 months from date of receipt, -20 to -70 °C as supplied;
1 week, 2 to 8 °C under sterile conditions;
Please avoid repeated freeze-thaw cycles.
1.Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterization of the streptococcal endopeptidase, IdeS, reveals that it is a cysteine protease with strict specificity for IgG cleavage due to exosite binding.[J].Biochemistry, 2004, 43(49):15540-15549.
2.Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615.
参考图片
M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing condition . The purity is greater than 95%.
M, marker;
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeS;
lane 3 rabbit IgG add 6.25 units IdeS;
lane 4 rabbit IgG add 8.3 units IdeS;
lane 5 rabbit IgG add 12.5 units IdeS;
lane 6 rabbit IgG add 25 units IdeS