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IdeS Protease

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IdeS Protease

品牌： UA BIOSCIENCE

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对比

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参数

分子量：

37.7kDa

纯度：

>95% by SDS-PAGE

产品介绍

产品信息

耦联标记

Unconjugated

氨基酸序列

N.A

分子量

37.7kDa

生物活性

40U/μl

酶活定义

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.

纯度

>95% by SDS-PAGE

性状

Liquid

表达系统

E.coli

缓冲体系

PBS pH 6.6, 50% glycerol

标签

His Tag

种属

Streptococcus pyogenes

电泳JSON

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M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing\ncondition . The purity is greater than 95%.

"}]

背景

别名

IdeS

背景

IdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcuspyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.

制备和贮存

溶解方法

N.A

保存方式

12 months from date of receipt, -20 to -70 °C as supplied;

1 week, 2 to 8 °C under sterile conditions;

Please avoid repeated freeze-thaw cycles.

文献

1.Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterization of the streptococcal endopeptidase, IdeS, reveals that it is a cysteine protease with strict specificity for IgG cleavage due to exosite binding.[J].Biochemistry, 2004, 43(49):15540-15549.

2.Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615.

参考图片

M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing condition . The purity is greater than 95%.

Results of 5μg rabbit IgG digestion separated under different quantity of IdeS, The reaction was incubated for8 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).

M, marker;

lane 1 rabbit IgG;

lane 2 rabbit IgG add 5 units IdeS;

lane 3 rabbit IgG add 6.25 units IdeS;

lane 4 rabbit IgG add 8.3 units IdeS;

lane 5 rabbit IgG add 12.5 units IdeS;

lane 6 rabbit IgG add 25 units IdeS

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货号：

UA070027-2000U

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2000U

5000U

2mg

1mg

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