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IdeZ Protease

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IdeZ Protease

品牌： UA BIOSCIENCE

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参数

分子量：

36kDa

纯度：

>95% by SDS-PAGE

产品介绍

产品信息

耦联标记

Unconjugated

氨基酸序列

N.A

分子量

36kDa

生物活性

10U/μL

酶活定义

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C

纯度

>95% by SDS-PAGE

性状

Liquid

表达系统

E.coli

缓冲体系

20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5

标签

His Tag

种属

Streptococcus pyogenes

电泳JSON

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\nM: marker; Complement IdeZ protease, 2μg on SDS-PAGE\nunder Non-reducing and reducing condition. The purity is greater than 95%\n\n\n\n

"}]

背景

别名

IgG endopeptidase

背景

IdeZ Protease secreted by the Streptococcus equi subspecies zooepidemicus. This product is an engineered recombinant protease over expressed in Escherichia coli. It can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and produce F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.

制备和贮存

溶解方法

N.A

保存方式

12 months from date of receipt, -20 to -70 °C as supplied;

1 week, 2 to 8 °C under sterile conditions;

Please avoid repeated freeze-thaw cycles.

文献

1.Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26.

2.Cheng-Wei H, Jie P, Yu-Qing O, et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J]. Chinese Journal of Preventive Veterinary Medicine, 2018.

参考图片

M: marker; Complement IdeZ protease, 2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%

Results of 5μg rabbit IgG digestion separated under different quantity of IdeZ protease, The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate)

M, marker:

lane 1 rabbit IgG;

lane 2 rabbit IgG add 5 units IdeZ;

lane 3 rabbit IgG add 10 units IdeZ;

lane 4 rabbit IgG add 33units IdeZ;

lane 5 rabbit IgG add 50 units IdeZ

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货号：

UA070028-4000U

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询价

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4000U

40000U

2mg

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