The XMG1.2 monoclonal antibody specifically binds to mouse interferon-γ (IFN-γ) protein. IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-γ production is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.
商品描述
XMG1.2
The XMG1.2 monoclonal antibody specifically binds to mouse interferon-γ (IFN-γ) protein. IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-γ production is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.
同种型
Rat IgG1, κ
克隆号
克隆 XMG1.2 (RUO)
浓度
0.2 mg/ml
产品详情
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
研发参考(3)
1. Cherwinski HM, Schumacher JH, Brown KD, Mosmann TR. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987; 166(5):1229-1244. (Biology).
2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry).
3. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Methodology: Flow cytometry).
参考图片
Flow cytometric analysis for IFN-γ in activated mouse splenocytes. Mouse Intracellular Cytokine-1 positive control cells (MiCK-1) offered by BD Biosciences as MN 554652, are activated mouse splenocytes prepared in the presence of a protein transport inhibitor. Fixed and permeabilized MiCK-1 cells were stained either with a PerCP-Cy™5.5 Rat IgG1, κ isotype control (left panel) or with the PerCP-Cy™5.5 Rat Anti-Mouse IFN-γ antibody (right panel). Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Flow cytometric analysis for IFN-γ in activated mouse splenocytes. Mouse Intracellular Cytokine-1 positive control cells (MiCK-1) offered by BD Biosciences as MN 554652, are activated mouse splenocytes prepared in the presence of a protein transport inhibitor. Fixed and permeabilized MiCK-1 cells were stained either with a PerCP-Cy™5.5 Rat IgG1, κ isotype control (left panel) or with the PerCP-Cy™5.5 Rat Anti-Mouse IFN-γ antibody (right panel). Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
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