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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
IgD
宿主
Rat IgG2a, κ
免疫原
Not reported
简单描述
The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all
Igh-C
haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells
in vitro
.
In vivo
injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.
商品描述
11-26c.2a
The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all
Igh-C
haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells
in vitro
.
In vivo
injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.
同种型
Rat IgG2a, κ
克隆号
克隆 11-26c.2a (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgD
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Hamilton AM, Lehuen A, Kearney JF. Immunofluorescence analysis of B-1 cell ontogeny in the mouse. Int Immunol. 1994; 6(3):355-361. (Biology).
2. Ishihara K, Wood WJ Jr, Wall R, et al. Multiple B29 containing complexes on murine B lymphocytes. Common and stage-restricted Ig-associated polypeptide chains. J Immunol. 1993; 150(6):2253-2262. (Biology).
3. Nitschke L, Kosco MH, Kohler G, Lamers MC. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl Acad Sci U S A. 1993; 90(5):1887-1891. (Clone-specific).
参考图片
Flow cytometric analysis of PE-conjugated anti-mouse IgD on mouse splenocytes. Isolated murine splenocytes were simultaneously stained with PE anti-mouse IgD (clone 11-26c.2a, Cat. No. 558597) and FITC anti-mouse CD3 (Clone 145-2C11, Cat. No. 553062) then analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur™ System.
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