The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all
Igh-C
haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells
in vitro
.
In vivo
injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
商品描述
11-26c.2a
The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all
Igh-C
haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes. Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells
in vitro
.
In vivo
injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
同种型
Rat IgG2a, κ
克隆号
克隆 11-26c.2a (RUO)
浓度
0.2 mg/ml
产品详情
BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV510
Violet 405 nm
327 nm, 405 nm
512 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgD
背景
别名
IGHD; Igh-5; Immunoglobulin heavy chain 5; Ig delta chain C region
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
数据库链接
Entrez-Gene ID
380797
参考图片
Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; Left Panel) or BD Horizon™ BV510 Rat anti-Mouse IgD antibody (Cat. No. 563110; Right Panel). Two-color flow cytometric dot plots showing the correlated expression patterns of IgD (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR™ II Flow Cytometer System.
Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either BD Horizon™ BV510 Rat IgG2a, κ Isotype Control (Cat. No. 562952; Left Panel) or BD Horizon™ BV510 Rat anti-Mouse IgD antibody (Cat. No. 563110; Right Panel). Two-color flow cytometric dot plots showing the correlated expression patterns of IgD (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR™ II Flow Cytometer System.
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