IgG Sepharose 6 Fast Flow affinity resin lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins. Designed for rapid, single-step purification of protein A and protein A fusion conjugates. Binds at least 2 mg protein A/mL resin. Suitable for tandem affinity purification (TAP) of protein complexes. Successful use in published research Applications using IgG Sepharose 6 Fast Flow include: Tandem affinity purification (TAP) to obtain functional proteins from human cells Tandem affinity purification to study phosphorylation sites on the Rpb1 C-terminal domain of RNA Polymerase II Isolating protein A-Cvt19 to study the cytoplasm to vacuole targeting (Cvt) pathway Compatible empty columns Cytiva offers a wide range of Life Sciences solutions for protein purification, including several types of empty columns. lgG Sepharose 6 Fast Flow is compatible with gravity flow, Tricorn, HiScale, and XK column lines. Convenient prepacked columns IgG Sepharose Fast Flow resin is available in the following format: HiTrap IgG Sepharose FF: convenient for easy use with a syringe, peristaltic pump, or chromatography system