Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb

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品牌: CST
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反应种属:
Rabbit
来源宿主:
Mouse
产品介绍
产品信息
荧光素标记
抗原名称
IgG
来源纯化

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

宿主
Mouse
简单描述

Affinity purified mouse anti-rabbit IgG (Conformation Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications.

商品描述

Product Usage Information

Recommended Antibody Dilution:1:2000

同种型
Mouse
应用
反应种属
Rabbit
目标/特异性

Specificity/Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations.

Species Reactivity:

Rabbit

背景
背景
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.
翻译后修饰
unmodified
制备和贮存
保存方式

-20°C

参考图片

Western blot analysis of decreasing concentrations of total rabbit IgG, reduced and denatured in 1X SDS loading buffer with DTT, using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 (Panel B), or Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected using Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.

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货号:
3678S
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100ul
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