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BD Pharmingen™ Biotin Goat Anti-Rabbit IgG
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Immunohistochemical staining of a formalin-fixed paraffin-embedded tissue section from normal human colon. Tissue sections were treated with Retrievagen A (Cat. No. 550524) and stained with Purified Rabbit Anti-Human CD88 (Cat. No. 550733) followed by Biotin Goat Anti-Rabbit IgG (Cat. No. 550338) and Streptavidin-HRP (Cat. No. 550946). For optimal indirect immunohistochemical staining, this antibody should be titrated (1:10 to 1:50 dilution) and visualized via Streptavidin-HRP together with the DAB detection system (Cat. No. 550880).
Flow cytometric analysis of CD88 expression on human peripheral blood granulocytes. Whole blood was stained with either Purified Rabbit IgG Isotype Standard (Cat. No. 550875; dashed line histogram) or Purified Rabbit Anti-Human CD88 (solid line histogram). CD88 (or Ig isotype standard) expression were visualized with Biotin Goat Anti-Rabbit IgG and PE Streptavidin (Cat. No. 554657). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.
Immunohistochemical staining of a formalin-fixed paraffin-embedded tissue section from normal human colon. Tissue sections were treated with Retrievagen A (Cat. No. 550524) and stained with Purified Rabbit Anti-Human CD88 (Cat. No. 550733) followed by Biotin Goat Anti-Rabbit IgG (Cat. No. 550338) and Streptavidin-HRP (Cat. No. 550946). For optimal indirect immunohistochemical staining, this antibody should be titrated (1:10 to 1:50 dilution) and visualized via Streptavidin-HRP together with the DAB detection system (Cat. No. 550880).
Flow cytometric analysis of CD88 expression on human peripheral blood granulocytes. Whole blood was stained with either Purified Rabbit IgG Isotype Standard (Cat. No. 550875; dashed line histogram) or Purified Rabbit Anti-Human CD88 (solid line histogram). CD88 (or Ig isotype standard) expression were visualized with Biotin Goat Anti-Rabbit IgG and PE Streptavidin (Cat. No. 554657). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.