BD Horizon™ BV480 Goat Anti-Rabbit IgG is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rabbit IgG primary antibodies. It reacts with rabbit IgG and the light chains of other rabbit immunoglobulins. BV480 Anti-Rabbit IgG has minimal crossreactivity with human, mouse, or rat serum proteins. As a second step staining reagent, it is reactive with rabbit polyclonal and monoclonal antibodies.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.
商品描述
Poly1272
BD Horizon™ BV480 Goat Anti-Rabbit IgG is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rabbit IgG primary antibodies. It reacts with rabbit IgG and the light chains of other rabbit immunoglobulins. BV480 Anti-Rabbit IgG has minimal crossreactivity with human, mouse, or rat serum proteins. As a second step staining reagent, it is reactive with rabbit polyclonal and monoclonal antibodies.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.
同种型
Goat Ig
克隆号
克隆 Polyclonal (RUO)
浓度
0.2 mg/ml
产品详情
BV480
The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV480
Violet 405 nm
440 nm
479 nm
应用
实验应用
Flow cytometry (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development)
反应种属
Rabbit (QC Testing)
目标/特异性
IgG
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS (Osteosarcoma, ATCC HTB-96) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized (5 min) with BD Phosflow™ Perm Buffer III (Cat. No. 558050), washed with 1x PBS, and blocked (30 min) with 5% Goat serum, 1% BSA, and 0.5% Triton™ X-100 diluted in 1x PBS. The cells were stained (1 hr) with Purified Rabbit Anti-PCNA antibody (Abcam) at 2.5 μg/mL. After washing, the cells were counterstained (45 min) in blocking buffer with the second step reagent, BD Horizon™ BV480 Anti- Rabbit IgG (Cat. No. 564879; 2.5 μg/mL; pseudo-colored green). Cells were then stained (1 hr) in blocking buffer with Alexa Fluor® 488 Mouse Anti-β-Tubulin (Cat. No. 558605; pseudo-colored red). BD Pharmingen DAPI Solution (Cat. No. 564907) was used as a nuclear counterstain (pseudo-colored blue). Slides were mounted with ProLong® Gold and the image was captured on a BD Pathway™ 435 Cell Analyzer (epifluorescence microscope) and merged using BD Attovision™ Software. 20X objective.
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