Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled Mouse IgG1
简单描述
The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.
商品描述
A85-1
The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 A85-1 (RUO)
浓度
0.2 mg/ml
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
IgG1
背景
别名
Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
研发参考(3)
1. Bhattacharya D, Lee DU, Sha WC. Regulation of Ig class switch recombination by NF-kappaB: retroviral expression of RelB in activated B cells inhibits switching to IgG1, but not to IgE. Int Immunol. 2002; 14(9):983-991. (Clone-specific: Flow cytometry).
2. Honjo T, Obata M, Yamawaki-Katoaka Y, et al. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene. Cell. 1979; 18(2):559-568. (Biology).
3. Ozaki K, Spolski R, Ettinger R, et al. Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004; 173(9):5361-5371. (Clone-specific: Flow cytometry).
数据库链接
Entrez-Gene ID
16017
参考图片
Flow cytometric analysis of CD22.2 expression by mouse splenocytes using PE Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were stained with either Purified Mouse IgG1, κ Anti-Mouse CD22.2 antibody (clone Cy34.1; solid line histogram) or with no antibody (for background staining control; dashed line histogram). After washing, the cells were stained with PE Rat Anti-Mouse IgG1 antibody (Cat. No. 550083) as the second step at 1 µg/test. The fluorescence histogram showing CD22.2 expression (or control staining) as detected by PE Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and analysis was performed using a BD™ LSR II Flow Cytometer System and FlowJo™ software.
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