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品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing)
实验应用：
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
产品介绍
产品介绍
产品信息
简单描述
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
商品描述
MOPC-21
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
同种型
Mouse IgG1, κ
克隆号
克隆 MOPC-21 (RUO)
产品详情
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
应用
实验应用
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
IgG1 kappa
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol to the cell pellet (30 minutes on ice or overnight at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer and stained with the Alexa Fluor® 488 Mouse IgG1, κ Isotype Control antibody (1 hour at RT; Cat. No. 557782). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
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