Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
产品介绍
产品介绍
产品信息
简单描述
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
商品描述
MOPC-21
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
同种型
Mouse IgG1, κ
克隆号
克隆 MOPC-21 (RUO)
产品详情
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
应用
实验应用
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
推荐用量
20 µl
目标/特异性
IgG1 kappa
背景
别名
IgG1, kappa; Ighg1, Igh-4, γg1; Igk-C, IGKC, Ig κ
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(1)
1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Clone-specific: Blocking).
参考图片
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol, (BD Phosflow™ Perm Buffer III, Cat. No. 558050) to the cell pellet (30 minutes on ice or overnight at at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer (Cat. No. 554656) and stained with Alexa Fluor® 647 Isotype Control antibody (1 hour at RT; Cat. No. 557783). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
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