The BD™ CompBead Plus Anti-Rat Ig, κ beads are polystyrene particles used to establish fluorescence compensation settings for multicolor flow cytometric analyses. The kit provides two types of particles, the BD™ CompBead Plus Anti-Rat Ig, κ particles, which bind any rat κ light chain-bearing immunoglobulin, and the BD™ CompBead Plus Negative Control (BSA) particles, which have no binding capacity. When mixed together with a fluorochrome-conjugated rat antibody, the BD™ CompBead Plus provides distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD™ CompBead Plus is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.
克隆号
(RUO)
BD化合物表
描述
数量/尺寸
零件号
EntrezGene ID
Negative Control (PBS with 1% BSA)
50 Tests (1 ea)
51-9006227
N/A
Anti-Rat Ig, κ
6 mL (1 ea)
51-9006320
N/A
应用
实验应用
Flow cytometry (Routinely Tested)
目标/特异性
Ig κ Compensation Bead
参考图片
H9 human embryonic stem cells (WiCell, Madison, WI) (hESCs), BD™ CompBeads (Cat. No. 552843), and BD™ CompBead Plus were stained with PE rat anti-SSEA-3 (Cat. No. 560237) and analyzed on a flow cytometer. Both unstained and stained hESCs are shown in blue, negative and stained BD™ CompBeads in green, and negative and stained BD™ CompBead Plus in red. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
BALB/c splenocytes, BD™ CompBeads (Cat. No. 552843), and BD™ CompBead Plus were stained with PE rat anti-mouse CD45R/B220 (Cat. No. 553089 ) and analyzed on a flow cytometer. Stained splenocytes are shown in blue, negative and stained BD™ CompBeads in green, and negative and stained BD™ CompBead Plus in red. Flow cytometry was performed on a BD LSR™ II flow cytometry system.
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