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BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a
品牌： BD Pharmingen
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对比
咨询反应种属：
Rat (Tested in Development)
来源宿主：
Mouse IgG2a
产品介绍
产品信息
荧光素标记
宿主
Mouse IgG2a
免疫原
Pooled Rat IgG
简单描述
The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
商品描述
G28-5
The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
同种型
Mouse IgG2a
克隆号
克隆 G28-5 (RUO)
浓度
0.2 mg/ml
产品详情
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
Violet 405 nm
407 nm
423 nm
应用
实验应用
Flow cytometry (Qualified)
反应种属
Rat (Tested in Development)
目标/特异性
IgG1, 2a
背景
别名
IgG1/IgG2a; Ig gamma-1/2a; Immunoglobulin gamma 1/2a
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
参考图片
Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Rat IgG1/IgG2a antibody (Cat. No. 743849; solid line histogram) for secondary staining of BALB/c splenocytes first stained with either Purified Isotype Control (dotted line histograms) or with Purified Rat IgG2a Anti-Mouse CD8a (solid line; left panel) or with Purified Rat IgG1 Anti-Mouse CD8b.2 antibody (solid line; right panel). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
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