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View LargerWhen Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 µg/mL, 100 µL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9315-HG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 1 mg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF Summary
Product Specifications
Pro110-Lys333
Analysis
Background: IgG1
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in human adult blood. It is composed of two heavy chains and two light chains. The variability of the IgG pool is generated by somatic recombination in B cells that have been exposed to foreign material. IgA, IgG, IgM, IgD, and IgD classes of human immunoglobulin (as well as the isotypes IgG1, IgG2a, IgG2b, and IgG3) are generated by class switch recombination within the heavy chain locus (1). Recombination within the light chain locus selects either kappa or lambda chains. Antibodies are secreted by plasma cells of the B lymphocyte lineage and constitute the humoral component of adaptive immunity (2, 3). Their variable regions bind to foreign antigens, while their Fc regions are recognized by Fc receptors expressed on macrophages, monocytes, neutrophils, NK cells, and CD8+ cytotoxic T cells (4). Phagocyte uptake of antibody-antigen complexes and antibody-opsonized pathogens is critical for clearance of the foreign material and immune homeostasis.
- Stavnezer, J. et al. (2008) Annu. Rev. Immunol. 26:261.
- Nutt, S.L. et al. (2015) Nat. Rev. Immunol. 15:160.
- Swanson, C.L. et al. (2013) Immunol. Res. 55:277.
- Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
参考图片
When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 μg/mL, 100 μL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.