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BD Horizon™ BV711 Rat IgG2b, κ Isotype Control
品牌： BD Pharmingen
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咨询实验应用：
Flow cytometry, Isotype control (Routinely Tested)
产品介绍
产品信息
荧光素标记
简单描述
The R35-38 hybridoma produces monoclonal rat IgG2b, κ. The target molecule specificity of this clone is unknown. R35-38 has little or no reactivity with mammalian cells and has been found useful as an immunoglobulin isotype control.
The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
商品描述
R35-38
The R35-38 hybridoma produces monoclonal rat IgG2b, κ. The target molecule specificity of this clone is unknown. R35-38 has little or no reactivity with mammalian cells and has been found useful as an immunoglobulin isotype control.
The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
同种型
Rat IgG2b, κ
克隆号
克隆 R35-38 (RUO)
浓度
0.2 mg/ml
产品详情
BV711
The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV711
Violet 405 nm
407 nm
713 nm
应用
实验应用
Flow cytometry, Isotype control (Routinely Tested)
目标/特异性
Rat IgG2b κ Isotype Ctrl
背景
别名
Rat IgG2b kappa Isotype Control
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
参考图片
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