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Horse IgM Whole Molecule - 008-0107
品牌： Rockland
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This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum and anti-Horse IgM (µ chain specific). No reaction was observed against anti-Horse IgG F(c). Some light chain cross reactivity will occur with anti-Horse IgG.
来源宿主：
Horse
产品介绍
产品介绍
产品信息
宿主
Horse
简单描述
Horse IgM Whole Molecule - 008-0107
商品描述
Horse IgM Whole Molecule - 008-0107
同种型
IgM
纯度
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum and anti-Horse IgM (µ chain specific). No reaction was observed against anti-Horse IgG F(c). Some light chain cross reactivity will occur with anti-Horse IgG.
浓度
0.89mg/mL by UV absorbance at 280 nm
形式
Liquid (sterile filtered)
防腐剂
0.1% (w/v) Sodium Azide
缓冲体系
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
产品类型
Native Protein
应用
实验应用
SDS-PAGE;Horse IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
目标/特异性
This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum and anti-Horse IgM (µ chain specific). No reaction was observed against anti-Horse IgG F(c). Some light chain cross reactivity will occur with anti-Horse IgG.
背景
别名
Horse IgM whole molecule
背景
Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Horse IgM whole molecule is ideal for investigators in Immunology, Microbiology, and Cell Biology.
制备和贮存
存储溶液
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
保存方式
Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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