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IL-1beta (D3U3E) Rabbit mAb
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IL-1beta (D3U3E) Rabbit mAb

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分子量:
17, 31
反应种属:
Human
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
IL-1beta
来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Simple Western™1:10 - 1:50
Immunofluorescence (Immunocytochemistry)1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized)1:100 - 1:400
同种型
Rabbit IgG
分子量
17, 31
研究领域
纤维化,免疫学和肿瘤学,神经科学
应用
反应种属
Human
目标/特异性

Specificity/Sensitivity

IL-1β (D3U3E) Rabbit mAb recognizes endogenous levels of total IL-1β protein. This antibody is not observed to detect endogenous levels of mature IL-1β. It can detect up to 100 pg of recombinant mature IL-1β.

Species Reactivity:

Human

敏感性
Endogenous
背景
背景
Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. 1.Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99. 2.Burns, K. et al. (1998) J Biol Chem 273, 12203-9. 3.Cao, Z. et al. (1996) Nature 383, 443-6. 4.Cao, Z. et al. (1996) Science 271, 1128-31. 5.Wesche, H. et al. (1997) Immunity 7, 837-47. 6.Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 7.Thornberry, N.A. et al. (1992) Nature 356, 768-74. 8.Cerretti, D.P. et al. (1992) Science 256, 97-100.
研究领域
纤维化,免疫学和肿瘤学,神经科学
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #18689.

数据库链接
Entrez-Gene ID
3553
UniProt ID
P01584

参考图片

Flow cytometric analysis of THP-1, untreated (blue, negative) or treated with LPS #14011 (100 ng/ml, 3 hr; green, positive) using IL-1β (D3U3E) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

Simple Western™ analysis of lysates (0.1 mg/mL) from THP-1 cells treated with TPA (80nM, O/N) + LPS (1 µg/ml 15min) using IL-1β (D3U3E) Rabbit mAb #12703. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

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货号:
12703S
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