IL-7 Protein, Human
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IL-7 Protein, Human

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    分子量:
    18-30 kDa (Reducing)
    18-30 kDa (Reducing)
    纯度:
    >95% by SDS-PAGE
    >95% by SDS-PAGE
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    氨基酸序列

    Asp26-His177 with C-terminal 6*His Tag

    DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEHHHHHHH

    分子量
    18-30 kDa (Reducing)
    纯度
    >95% by SDS-PAGE
    内毒素水平
    <1EU/mg
    性状
    Lyophilized powder
    表达系统
    HEK293
    缓冲体系
    10 mM PBS, pH 7.4.
    标签
    His Tag
    种属
    Human
    电泳JSON
    [{"imgUrl":"https://univ-pmc.oss-cn-shanghai.aliyuncs.com/product.imager.attribute/0/0a136ca4-710b-4892-b973-7fd98dcc02a2.png","description":"

    2μg (R: reducing conditions, N: non-reducing conditions).

    "}]
    背景
    别名
    Interleukin-7
    背景

    Interleukin 7(IL-7)is a mature protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4kDa. Recombinant human IL-7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells.

    IL-7 is a proteinaceous biological response modifier that has a bioactive tertiary structure dependent on disulfide bond formation.

    IL-7-stimulated pro-B cells partially differentiated into a pre-B cell population, on the basis of the loss of CD34 and terminal deoxynucleotidyl (TdT) expression, and the acquisition of cytoplasmic mu heavy chains.

    制备和贮存
    溶解方法
    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
    保存方式

    · 12 months from date of receipt, -20 to -70 °C as supplied.

    · 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    · 1 week, 2 to 8 °C under sterile conditions after reconstitution. 

    · Please avoid repeated freeze-thaw cycles.

    文献
    文献

    1. Proc Natl Acad Sci U S A. 1989 Jan;86(1):302-6.
    2. J Biol Chem. 1997 Dec 26;272(52):32995-3000.
    3. J Immunol. 1995 Jan 1;154(1):58-67.

    数据库链接
    Accession

    参考图片

    2μg (R: reducing conditions, N: non-reducing conditions).

    Measured in a cell proliferation assay using 2E8 cells. The EC50 for this effect is less than 1.5ng/ml.

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    货号:
    UA040118-1mg
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