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- 描述数量/尺寸零件号EntrezGene ID
- Human IL-10 Capture Bead B71 Vial(s) (1 ea)51-9005291N/A
- Human IL-10 PE Detection ReagentN/A51-9004043N/A
- Human IL-10 Standard1 Vial(s) (2 ea)51-9003501N/A
参考图片
Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.
Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.
Figure 1. Example BD CBA Human IL-10 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.
Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.
Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.
Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Human IL-10 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.