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rhIL-17A, CF (50 ug)
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View LargerRecombinant Human IL-17 (Catalog # 317-ILB) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.4-4 ng/mL.
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
317-ILB
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 100 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Recombinant Human IL-17A Protein, CF Summary
Product Specifications
Ile20-Ala155, with an N-terminal Met
Analysis
Background: IL-17/IL-17A
Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).
- Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
- Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
- Yao, Z. et al. (1995) J. Immunol. 155:5483.
- Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
- Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
- Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
- Wright, J.F. et al. (2008) J. Immunol. 181:2799.
- Rong, Z. et al. (2009) Cell Res. 19:208.
- Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
- Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
- Liang, S.C. et al. (2007) J. Immunol. 179:7791.
- O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
- Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
- Hsu, H. et al. (2008) Nat. Immunol. 9:166.
- Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
- Wang, L. et al. (2009) J. Exp. Med. 206:1457.
- Kryczek, I. et al. (2009) Blood 114:357.