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- 描述数量/尺寸零件号EntrezGene ID
- Human IL-17A StandardN/A51-9006068N/A
- Human IL-17A PE Detection ReagentN/A51-9006081N/A
- Human IL-17A Capture Bead B5100 Tests (1 ea)51-9006083N/A
参考图片
Figure 1. Example BD CBA Human IL-17A Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.
Recovery: Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.
Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human IL-17A Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).
Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.