首页
rmIL-19, CF (25 ug)
rmIL-19, CF (25 ug)

rmIL-19, CF (25 ug)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    产品介绍
    产品信息

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    2915-IL

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    2915-IL/CF

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Mouse IL-19 Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 20.0-200 pg/mL.
    Source
    E. coli-derived mouse IL-19 protein
    Leu25-Ala176, with an N-terminal Met
    Accession #
    Q8CJ70
    N-terminal Sequence
    Analysis
    Met
    Predicted Molecular Mass
    17.7 kDa
    背景
    背景

    Background: IL-19

    Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2 - 4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35 - 45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

    References
    1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
    2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
    3. Zdanov, (2004) Curr. Pharm. Des. 10:3873. 
    4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
    5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
    6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
    7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
    8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
    9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
    10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
    11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
    13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
    14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
    15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.
    Long Name
    Interleukin 19
    Entrez Gene IDs
    29949 (Human); 329244 (Mouse)
    Alternate Names
    IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    2915-IL-025/CF
    一键复制
    询价
    25ug
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价