IL-1beta (3A6) Mouse mAb

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IL-1beta (3A6) Mouse mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

IL-1beta

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant human IL-1β protein.

宿主

Mouse

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunohistochemistry (Paraffin)	1:50 - 1:200

同种型

Mouse IgG1

分子量

17,31

研究领域

纤维化,免疫学和肿瘤学,神经科学

应用

反应种属

Human,Mouse,

目标/特异性

Specificity/Sensitivity

IL-1β (3A6) Mouse mAb recognizes endogenous levels of total IL-1β protein.

Species Reactivity:

Human, Mouse

敏感性

Endogenous

背景

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. 1.Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99. 2.Burns, K. et al. (1998) J Biol Chem 273, 12203-9. 3.Cao, Z. et al. (1996) Nature 383, 443-6. 4.Cao, Z. et al. (1996) Science 271, 1128-31. 5.Wesche, H. et al. (1997) Immunity 7, 837-47. 6.Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 7.Thornberry, N.A. et al. (1992) Nature 356, 768-74. 8.Cerretti, D.P. et al. (1992) Science 256, 97-100.

研究领域

纤维化,免疫学和肿瘤学,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #27989.

数据库链接

Entrez-Gene ID

3553

UniProt ID

P01584

参考图片

Immunohistochemical analysis of paraffin embedded THP-1 cell pellets, control (left) or LPS-treated (right) using IL-1-β (3A6) Mouse mAb.

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/mL, 3 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +) and LPS (100 ng/mL, 6 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of 1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 and 1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β (3A6) Mouse mAb.

Simple Western™ analysis of lysates (1.0 mg/mL) from THP-1 cells treated with TPA (80nM, O/N) and LPS (1ug/ml, 1 hr) using IL-1β (3A6) Mouse mAb #12242. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 2-40 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human large intestine (chronic colitis of the colon) using IL-1-β (3A6) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human large intestine (ulcerative chronic colitis of the rectum) using IL-1-β (3A6) Mouse mAb.

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货号：

12242S

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