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rhIL-1b (25 ug)
rhIL-1b (25 ug)
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rhIL-1b (25 ug)

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品牌： R&D Systems
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    产品介绍
    产品信息

    Scientific Data

    SEC-MALS View Larger

    Recombinant Human IL-1beta Protein (Catalog # 201-LB) has a molecular weight (MW) of 17.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

    Bioactivity Recombinant Human IL-1 beta/IL-1F2 Protein BioactivityView Larger

    Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

    SDS-PAGE Recombinant Human IL-1 beta/IL-1F2 Protein SDS-PAGEView Larger

    1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    201-LB

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    ReconstitutionReconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    *The 1 mg pack size (01M) can be stored for 1 month at 2 to 8 °C under sterile conditions after opening.

    201-LB/CF

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
    ReconstitutionReconstitute at 50-200 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:


    Recombinant Human IL-1 beta/IL-1F2 Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
    Source
    E. coli-derived human IL-1 beta/IL-1F2 protein
    Ala117-Ser269
    Accession #
    NP_000567
    N-terminal Sequence
    Analysis
    Ala117 & Pro118
    Predicted Molecular Mass
    17 kDa
    SDS-PAGE
    17 kDa, reducing conditions
    背景
    别名
    catabolin,IL1 beta,IL-1 beta,IL-1,IL1B,IL-1b,IL1-BETA,IL-1F2,IL1F2IL-1 beta,interleukin 1, beta,interleukin-1 beta,preinterleukin 1 beta,pro-interleukin-1-beta,Interleukin 1 beta
    背景

    Background: IL-1 beta/IL-1F2

    IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

    References
    1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
    2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
    4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
    5. March, C.J. et al. (1985) Nature 315:641.
    6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
    7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
    Long Name
    Interleukin 1 beta
    Entrez Gene IDs
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
    Alternate Names
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is typically 12 pg/mL.

    1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

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    货号：
    201-LB-025
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