BD™ Cytometric Bead Array (CBA) Mouse IL-1β Flex Set

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse IL-1β Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Example BD CBA Mouse IL-1β Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse IL-1β Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.