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BD OptEIA™ Human IL-2 ELISA Set
品牌： BD Pharmingen
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对比
咨询反应种属：
Human (QC Testing)
实验应用：
ELISA (Routinely Tested)
产品介绍
产品介绍
产品信息
抗原名称
IL-2
克隆号
(RUO)
BD化合物表
- 描述数量/尺寸零件号EntrezGene ID
- Recombinant Human IL-2 Lyophilized StandardN/A51-26276E3558
- Detection Antibody Biotin Anti-Human IL-2N/A51-26272E3558
- Capture Antibody Purified Anti-Human IL-2N/A51-26271E3558
- Enzyme Reagent Streptavidin-horseradish peroxidase conjugate (SAv-HRP)N/A51-9002813N/A
应用
实验应用
ELISA (Routinely Tested)
反应种属
Human (QC Testing)
目标/特异性
IL-2
文献
文献
研发参考(12)
1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology).
2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology).
3. Andersson J, Abrams J, Bjork L, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994; 83(1):16-24. (Biology).
4. Fernandez V, Andersson J, Andersson U, Troye-Blomberg M. Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid. Eur J Immunol. 1994; 24(8):1808-1815. (Biology).
5. Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Biology).
6. Meager A. Characterization of interferons and immunoassays. In: Clemens MJ, Morris AG, Gearing AJH, ed. Lymphokines and Interferons. A Practical Approach. Oxford: IRL Press Ltd; 1987:105-127.
7. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology).
8. Smith, KA. Interleukin-2: inception, impact, and implications. Science. 1988; 240(4856):1169-1176. (Biology).
9. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. (Biology).
10. Stern AS, Pan YC, Urdal DL, et al. Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia. Proc Natl Acad Sci U S A. 1984; 81(3):871-875. (Biology).
11. Taniguchi T, Matsui H, Fujita T, et al. Structure and expression of a cloned cDNA for human interleukin-2. Nature. 1983; 302(5906):305-310. (Biology).
12. Verdier F, Aujoulat M, Condevaux F, Descotes J. Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys. Toxicology. 1995; 105(1):81-90. (Biology).
参考图片
This standard curve is fordemonstration only. A standard curve must be run with each assay. \"Typical Standard Curve\" and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing.
Serial dilutions within the plate may also be performed by pipetting 100 µL of Assay Diluent into each standard well except the highest (500 pg/mL), then adding 100 µL of the 500 pg/mL standard to both that well and the 250 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 µL of the 250 pg/mL standard to the 125 pg/mL well. Continue these dilutions to the 7.8 pg/mL standard well, out of which the extra 100 ìL should be discarded.
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