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hIL-3 MAb (Cl 4806) (25 ug)
hIL-3 MAb (Cl 4806) (25 ug)
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hIL-3 MAb (Cl 4806) (25 ug)

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品牌： R&D Systems
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    来源宿主：
    Mouse IgG1
    Mouse IgG1
    实验应用：
    Western Blot(1 µg/mL)
    Immunocytochemistry(8-25 µg/mL)
    Western Blot(1 µg/mL)
    Immunocytochemistry(8-25 µg/mL)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from ascites
    抗原名称
    Hematopoietic growth factor， IL3， IL-3， IL-3MGC79398， interleukin 3 (colony-stimulating factor, mult
    宿主
    Mouse IgG1
    免疫原
    E. coli-derived recombinant human IL-3
    Ala20-Phe152
    Accession # Q6GS87
    克隆号
    4806

    Scientific Data

    Neutralization Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 Antibody.View Larger

    Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 Antibody. Recombinant Human IL‑3 (Catalog # 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND50 is typically 0.03-0.08 µg/mL.

    Immunocytochemistry IL-3 antibody in Human PBMCs by Immunocytochemistry (ICC).View Larger

    IL‑3 in Human PBMCs. IL-3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-3 Monoclonal Antibody (Catalog # MAB203) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

    Human IL-3 Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human IL-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse IL-3 is observed. Does not neutralize the biological activity of recombinant mouse IL-3.
    Source
    Monoclonal Mouse IgG1 Clone # 4806
    Purification
    Protein A or G purified from ascites
    Immunogen
    E. coli-derived recombinant human IL-3
    Ala20-Phe152
    Accession # Q6GS87
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Western Blot
    1 µg/mL
    Recombinant Human IL‑3 (Catalog # 203-IL)
    Immunocytochemistry
    8-25 µg/mL
    See below
    Neutralization
    Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.03-0.08 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3.

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot(1 µg/mL)
    Immunocytochemistry(8-25 µg/mL)
    背景
    别名
    Hematopoietic growth factor,IL3,IL-3,IL-3MGC79398,interleukin 3 (colony-stimulating factor, multiple),interleukin-3,Mast cell growth factor,mast-cell growth factor,MCGF,MCGFMGC79399,MULTI-CSF,multilineage-colony-stimulating factor,Multipotential colony-stimulating factor,P-cell stimulating factor,P-cell-stimulating factor,Interleukin 3
    背景

    Background: IL-3

    Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

    IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

    Long Name
    Interleukin 3
    Entrez Gene IDs
    3562 (Human); 16187 (Mouse); 24495 (Rat)
    Alternate Names
    Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 Antibody. Recombinant Human IL‑3 (Catalog # 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND50 is typically 0.03-0.08 µg/mL.

    IL‑3 in Human PBMCs. IL‑3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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    货号：
    MAB203-SP
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