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产品介绍
产品介绍
产品信息
来源纯化
Recombinant mouse IL-33 was expressed in E. coli and is supplied in a lyophilized form.
宿主
E. coli
简单描述
| MW (kDa) | 17.7 |
| Purity | A greater than or equal to 90% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-33. |
| Activity | The bioactivity of recombinant mIL-33 was determined in a D10S cell proliferation assay. The ED50 of each lot is less than or equal to 2 ng/ml. |
分子量
17.7
研究领域
纤维化,神经科学,
应用
反应种属
Mouse
背景
背景
IL-33 (Interleukin-33, NF-HEV) is a nuclear cytokine that is part of the IL-1 family (1,2). Originally discovered as the ‘nuclear factor from high endothelial venules,’ it is widely expressed by endothelial cells as well as epithelial barrier tissues (1,5). IL-33 is involved in regulating type 2-associated innate immune responses (2,4). It is the ligand for ST2 which, along with IL-1RAcP, forms the IL-33R signaling complex (2,3). IL-33 functions as an alarmin upon cellular damage or stress, and is associated with allergic inflammation and asthma (5,6).
1.Baekkevold, E.S. et al. (2003) Am J Pathol 163, 69-79.
2.Schmitz, J. et al. (2005) Immunity 23, 479-90.
3.Chackerian, A.A. et al. (2007) J Immunol 179, 2551-5.
4.Smithgall, M.D. et al. (2008) Int Immunol 20, 1019-30.
5.Moussion, C. et al. (2008) PLoS One 3, e3331.
6.Cayrol, C. and Girard, J.P. (2014) Curr Opin Immunol 31, 31-7.
研究领域
纤维化,神经科学,
制备和贮存
保存方式
Mouse IL-33 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
数据库链接
Entrez-Gene ID
77125
UniProt ID
Q8BVZ5
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