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h/mIL-5 MAb (Cl TRFK (25 ug)

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h/mIL-5 MAb (Cl TRFK (25 ug)

品牌： R&D Systems

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产品介绍

产品信息

荧光素标记

Unconjugated

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

BCDF mu， B-cell differentiation factor I， BCGFII， EDF， Eo-CSF， Eosinophil differentiation factor， IL

宿主

Rat IgG1

免疫原

Mouse T cell clone-derived partially purified recombinant mouse IL-5

克隆号

TRFK5

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-5 antibody in Human PBMCs antibody by Flow Cytometry.

View Larger

Detection of IL‑5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B) and Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization

View Larger

Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND₅₀ is typically 0.004-0.015 µg/mL.

Human/Mouse IL-5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.

Source

Monoclonal Rat IgG₁ Clone # TRFK5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse T cell clone-derived partially purified recombinant mouse IL-5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Mouse IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL‑5 Biotinylated Antibody (Catalog # BAM705)

Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)

Human IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL‑5 Biotinylated Antibody (Catalog # BAM6051)

Standard: Recombinant Human IL-5 Protein (Catalog # 205-IL)

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.004-0.015 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

CyTOF-reported, Intracellular Staining by Flow Cytometry, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization

背景

别名

BCDF mu,B-cell differentiation factor I,BCGFII,EDF,Eo-CSF,Eosinophil differentiation factor,IL5,IL-5,IL-5T-cell replacing factor,interleukin 5 (colony-stimulating factor, eosinophil),interleukin-5,TRF,TRFB cell differentiation factor I,Interleukin 5

背景

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name

Interleukin 5

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Alternate Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

研究领域

Related Research Areas

制备和贮存

溶解方法

Reconstitute at 0.5 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of IL‑5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂ Secondary Antibody (Catalog # F0105B) and Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody. Recombinant Mouse IL‑5 (Catalog # 405-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405). The ND₅₀ is typically
0.004-0.015 µg/mL.

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货号：

MAB405-SP

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