h/mIL-5 MAb (Cl TRFK (25 ug)

品牌： R&D Systems

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产品介绍

产品信息

荧光素标记

Unconjugated

纯化方式

Protein A or G purified from cell culture supernatant

抗原名称

BCDF mu， B-cell differentiation factor I， BCGFII， EDF， Eo-CSF， Eosinophil differentiation factor， IL

宿主

Rat IgG1

免疫原

Mouse T cell clone-derived partially purified recombinant mouse IL-5

克隆号

TRFK5R

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405R). The ND₅₀ is typically 4-15 ng/mL.

Human/Mouse IL-5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.

Source

Recombinant Monoclonal Rat IgG₁ Clone # TRFK5R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse T cell clone-derived partially purified recombinant mouse IL-5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL‑5 Biotinylated Antibody (Catalog # BAM6051)

Standard: Recombinant Human IL-5 Protein (Catalog # 205-IL)

Mouse IL-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL‑5 Biotinylated Antibody (Catalog # BAM705)

Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 4-15 ng/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization

背景

别名

BCDF mu,B-cell differentiation factor I,BCGFII,EDF,Eo-CSF,Eosinophil differentiation factor,IL5,IL-5,IL-5T-cell replacing factor,interleukin 5 (colony-stimulating factor, eosinophil),interleukin-5,TRF,TRFB cell differentiation factor I,Interleukin 5

背景

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name

Interleukin 5

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Alternate Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

研究领域

Related Research Areas

制备和贮存

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405R). The ND₅₀ is typically 4-15 ng/mL.

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货号：

MAB405R-SP

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